#Alternatives to snapgene pdf#
Sanger sequencing of colony-based PCR products will result in chromatogram files and sequence (or fasta) files.Ĭhromatogram files in standard PDF format can be viewed and fasta files trimmed in a text editor.
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19Įfficient cleavage requires at least two copies of the NmeAIII recognition sequence.Analysis of colony-based PCR 16s rRNA data Sticky ends from different AlwNI sites may not be compatible. PciI is inhibited by nonionic detergents.Īfter cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility. Sticky ends from different AflIII sites may not be compatible. Sticky ends from different BsmI sites may not be compatible.
PaeR7I does not recognize the sequence CTCTCGAG.īsiWI is typically used at 55☌, but is 50% active at 37☌.īssHII is typically used at 50☌, but is 75% active at 37☌. Sticky ends from different Esp3I sites may not be compatible. Sticky ends from different BsmBI sites may not be compatible.īsmBI-v2 is an improved version of BsmBI. Sticky ends from different BtgI sites may not be compatible.Īfter cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.ĮcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
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